通過鈍化global regulator FruR調整大腸桿菌中碳流量來提高色氨酸的產量
大腸桿菌中，果糖抑制劑（FruR）通過中心代謝途徑影響碳的流量。這篇研究中，FB-04大腸桿菌通過敲除了FruR基因從而使FruR失活使色氨酸的產量增加。基因敲除的FB-04大腸桿菌（△fruR）不僅展現出更高的生長效率而且還大幅度提升了色氨酸的產量。FB-04（△FruR）大腸桿菌中色氨酸的產量和每分子葡萄糖產生的色氨酸相比于沒有敲除的大腸桿菌分別增加了65.5%和52.4%。代謝組學分析，顯示基因敲除的大腸桿菌顯著提高了糖酵解、戊糖磷酸途徑和三羧酸循環代謝，用于生物合成色氨酸的前體和基質水平升高。在不考慮遺傳背景下，fruR的缺失會升高色氨酸的產量并提高碳源的利用效率。
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文獻內容
Title：Modulating the direction of carbon ?ow in Escherichia coli to improve 1-tryptophan production by inactivating the global regulator FruR
Author：Lina Liua,b, Xuguo Duana,b, Jing Wua,b,∗
Abstract：
The fructose repressor (FruR) affects carbon ?ux through the central metabolic pathways of Escherichia coli. In this study, l-tryptophan production in Escherichia coli FB-04 was improved by knocking out the fruR gene, thereby inactivating FruR. This fruR knockout strain, E. coli FB-04(L1fruR), not only exhibited higher growth ef?ciency, it also showed substantially improved l-tryptophan production. l-tryptophan production by E. coli FB-04(L1fruR) and l-tryptophan yield per glucose were increased by 62.5% and 52.4%, respectively, compared with the parent E. coli FB-04. Metabolomics analysis showed that the fruR knockout signi?cantly enhances metabolic ?ow through glycolysis, the pentose phosphate pathway and the TCA cycle, increasing levels of critical precursors and substrates for l-tryptophan biosynthesis. These results indicate that fruR deletion should enhance l-tryptophan production and improve the ef?ciency of carbon source utilization independent of genetic background.
