游離不飽和脂肪酸描繪肥胖個體代謝狀態的研究
肥胖，常常是心血管疾病、2型糖尿病等代謝性疾病的重要病因，本篇文獻研究發現體重正常的健康者和健康的肥胖者血清中的游離脂肪酸譜非常相似，但是患有糖尿病的肥胖者的血清樣本中游離脂肪酸顯著性升高，其中不飽和脂肪酸更為突出。又以C20：3n-6最為顯著。在后期10年隨訪樣本收集研究中發現那些后來發現糖尿病病變的肥胖者的不飽和脂肪酸發生了顯著性變化，這些變化遠遠早于血糖和胰島素等目前臨床指標的變化。在接受代謝手術治療后，這些指標明細降低，并且，術前的不飽和脂肪酸水平的水平可以預測術后2年內糖尿病的復發情況。
文章中提到的技術難點是游離脂肪酸含量較低且數量較多，如何一次性同時檢測、絕對定量這些游離脂肪酸是需要解決的重要問題之一，譜領生物已開發成熟、完善的，從C2到C24包括三十多種短鏈、中長鏈游離脂肪酸的絕對定量方案，并對外提供服務，已為多位客戶提供服務并獲得好評。詳情請見脂肪酸檢測分析。
文獻內容
Title:Circulating Unsaturated Fatty Acids Delineate the Metabolic Status of Obese Individuals.
Author：Yan Ni, Linjing Zhao, Haoyong Yu, Xiaojing Ma, Yuqian Bao, Cynthia Rajani, Lenora W.M. Loo, Yurii B. Shvetsov, Herbert Yu, Tianlu Chen, Yinan Zhang, Congrong Wang, Cheng Hu, Mingming Su, Guoxiang Xie, Aihua Zhaoa Wei Jia, and Weiping Jia
Journal: EBioMedicine. 2015 Oct; 2(10): 1513–1522.
Keywords: Free fatty acids, Metabolic syndrome, Obesity, Unsaturated fatty acids, Type 2 diabetes, Insulin resistance.
Abstract：
Background
Obesity is not a homogeneous condition across individuals since about 25–40% of obese individuals can maintain healthy status with no apparent signs of metabolic complications. The simple anthropometric measure of body mass index does not always reflect the biological effects of excessive body fat on health, thus additional molecular characterizations of obese phenotypes are needed to assess the risk of developing subsequent metabolic conditions at an individual level.
Methods
To better understand the associations of free fatty acids (FFAs) with metabolic phenotypes of obesity, we applied a targeted metabolomics approach to measure 40 serum FFAs from 452 individuals who participated in four independent studies, using an ultra-performance liquid chromatograph coupled to a Xevo G2 quadruple time-of-flight mass spectrometer.
Findings
FFA levels were significantly elevated in overweight/obese subjects with diabetes compared to their healthy counterparts. We identified a group of unsaturated fatty acids (UFAs) that are closely correlated with metabolic status in two groups of obese individuals who underwent weight loss intervention and can predict the recurrence of diabetes at two years after metabolic surgery. Two UFAs, dihomo-gamma-linolenic acid and palmitoleic acid, were also able to predict the future development of metabolic syndrome (MS) in a group of obese subjects.
Interpretation
These findings underscore the potential role of UFAs in the MS pathogenesis and also as important markers in predicting the risk of developing diabetes in obese individuals or diabetes remission after a metabolic surgery.
