基于GC-MS對儲藏條件不同的商業馬鈴薯品種塊莖對的代謝組學研究
本篇論文的內容主要講述利用氣質聯用作為檢測氨基酸，有機酸，糖類，糖醇類和脂肪酸的一個平臺，通過針對不同的后代（幼苗）、淀粉含量、烹飪方式和休眠期的檢測得到的結果來描述六種不同品種商業馬鈴薯品種（Hópehely, Katica, Lorett, Somogyi kifli, Vénusz Gold, and White Lady）的特征。分析方法是氣質聯用平臺檢測到代謝物之后，采用主成分分析去掉無關的代謝物，得到增加和減少的代謝物的含量，再對比與其對應的馬鈴薯品種，最終分析得到結果。
這篇文章中使用的代謝組學技術譜領生物已開發成熟、完善，并對外提供服務，已為多位客戶提供服務并獲得好評。
文獻內容
Title:A GC–MS-based metabolomics study on the tubers of commercial potato cultivars upon storage
Author: Csilla Uri , Zsófia Juhász , Zsolt Polgár , Zsófia Bánfalvi .
Journal: Food Chemistry
Keywords: GC–MS; Metabolomics; Solanum tuberosum; Storage; Tuber.
Abstract：
Using gas chromatography–mass spectrometry (GC–MS) as a system for the detection of amino acids, organic acids, sugars, sugar alcohols, and fatty acids, we characterised six commercial potato cultivars (Hópehely, Katica, Lorett, Somogyi kifli, Vénusz Gold, and White Lady) with different pedigrees, starch contents, cooking types, and dormancy periods, in five developmental stages from harvest to sprouting. The tubers were stored at 20–22 °C in the dark. The metabolite data were subjected to principal component analysis. No correlation between metabolite contents of freshly harvested tubers and starch content or cooking type of the cultivars was detected. The storage decreased the fructose and sucrose and increased the proline concentrations of tubers. Irrespective of the length of dormancy a substantial difference in metabolite composition at each time point upon storage was detected in each cultivar except Somogyi kifli, the only cultivar amongst those tested with a pure Solanum tuberosum origin and A cooking type.
